{"id":2572,"date":"2025-10-17T10:25:15","date_gmt":"2025-10-17T02:25:15","guid":{"rendered":"https:\/\/bio-pal.com\/eng\/?p=2572"},"modified":"2025-10-17T10:31:20","modified_gmt":"2025-10-17T02:31:20","slug":"fast-library-prep-kit","status":"publish","type":"post","link":"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/","title":{"rendered":"Fast Library Prep Kit"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_81 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 eztoc-toggle-hide-by-default' ><li class='ez-toc-page-1 ez-toc-heading-level-5'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Fast_Library_Prep_Kit\" >Fast Library Prep Kit<\/a><ul class='ez-toc-list-level-6' ><li class='ez-toc-heading-level-6'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Overview\" >Overview<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-6'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Advantages\" >Advantages<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-5'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Performance\" >Performance<\/a><ul class='ez-toc-list-level-6' ><li class='ez-toc-heading-level-6'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Superior_Performance_for_Low_Input_Amount_Samples\" >Superior Performance for Low Input Amount Samples<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-6'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Excellent_Performance_for_Low-quality_Samples\" >Excellent Performance for Low-quality Samples<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-5'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Higher_Molecular_Recovery_Rate\" >Higher Molecular Recovery Rate<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-5'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#High_Repeatability_for_Stable_Performance\" >High Repeatability for Stable Performance<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-5'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#High_Accuracy_for_Low-Frequency_Mutation_Detection\" >High Accuracy for Low-Frequency Mutation Detection<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-5'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/bio-pal.com\/eng\/fast-library-prep-kit\/#Ordering_Info\" >Ordering Info<\/a><\/li><\/ul><\/nav><\/div>\n<h5 class=\"title title-font mb25 wow fadeInDown animated\"><span class=\"ez-toc-section\" id=\"Fast_Library_Prep_Kit\"><\/span>Fast Library Prep Kit<span class=\"ez-toc-section-end\"><\/span><\/h5>\n<h6><\/h6>\n<h6 class=\"common-title left mb25 fz36\"><span class=\"ez-toc-section\" id=\"Overview\"><\/span>Overview<span class=\"ez-toc-section-end\"><\/span><\/h6>\n<div class=\"richtext table mb25\">The IGT\u00ae Fast Library Prep Kit v2.0 is a rapid library construction kit for fragmented dsDNA. It offers a fast and user-friendly alternative to a quick 16S <a title=\"NGS Kit\" href=\"https:\/\/bio-pal.com\/eng\/category\/products\/ngs-reagents-kits\/\" target=\"_blank\" rel=\"noopener\">NGS library prep kit<\/a>, especially for applications based on A-T ligation, with a simple workflow and suitable input range from 1 ng to 500 ng.<\/div>\n<div>\n<div id=\"detail2\" class=\"sep-detail-overview\">\n<div class=\"sep-container\">\n<div class=\"item mb50 wow fadeInUp animated\">\n<h6><\/h6>\n<h6 class=\"common-title left mb25 fz36\"><span class=\"ez-toc-section\" id=\"Advantages\"><\/span>Advantages<span class=\"ez-toc-section-end\"><\/span><\/h6>\n<div class=\"richtext table mb25\">\n<ul class=\" list-paddingleft-2\">\n<li>Suitable for low input amount and low-quality samples.<\/li>\n<li>Simple and rapid workflow, only requires 3.5 h to finish the library construction.<\/li>\n<li>Combined with self-developed UDI and UMI technologies, it can effectively reduce data crosstalk and meet the needs of low-frequency variation detection.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<h5><\/h5>\n<h5 class=\"item mb50 wow fadeInUp animated\"><span class=\"ez-toc-section\" id=\"Performance\"><\/span>Performance<span class=\"ez-toc-section-end\"><\/span><\/h5>\n<h6><span class=\"ez-toc-section\" id=\"Superior_Performance_for_Low_Input_Amount_Samples\"><\/span>Superior Performance for Low Input Amount Samples<span class=\"ez-toc-section-end\"><\/span><\/h6>\n<p>The IGT\u00ae Fast Library Prep Kit v2.0 has a high library conversion rate that leads to a higher library yield even with low input amount compared to major competitors.<\/p>\n<figure id=\"attachment_2573\" aria-describedby=\"caption-attachment-2573\" style=\"width: 698px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2573\" src=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/superior-performance-for-low-input-amount-samples.webp\" alt=\"superior-performance-for-low-input-amount-samples\" width=\"698\" height=\"334\" srcset=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/superior-performance-for-low-input-amount-samples.webp 698w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/superior-performance-for-low-input-amount-samples-300x144.webp 300w\" sizes=\"auto, (max-width: 698px) 100vw, 698px\" \/><figcaption id=\"caption-attachment-2573\" class=\"wp-caption-text\">superior-performance-for-low-input-amount-samples<\/figcaption><\/figure>\n<h6><span class=\"ez-toc-section\" id=\"Excellent_Performance_for_Low-quality_Samples\"><\/span>Excellent Performance for Low-quality Samples<span class=\"ez-toc-section-end\"><\/span><\/h6>\n<p>Compared to major competitors, the IGT\u00ae Fast Library Prep Kit v2.0 can effectively improve the library quality even with the low-quality samples like FFPE, so as to obtain reliable sequencing results.<\/p>\n<figure id=\"attachment_2574\" aria-describedby=\"caption-attachment-2574\" style=\"width: 1448px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2574\" src=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Excellent-Performance-for-Low-quality-Samples.webp\" alt=\"Excellent-Performance-for-Low-quality-Samples\" width=\"1448\" height=\"355\" srcset=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Excellent-Performance-for-Low-quality-Samples.webp 1448w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Excellent-Performance-for-Low-quality-Samples-300x74.webp 300w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Excellent-Performance-for-Low-quality-Samples-1024x251.webp 1024w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Excellent-Performance-for-Low-quality-Samples-768x188.webp 768w\" sizes=\"auto, (max-width: 1448px) 100vw, 1448px\" \/><figcaption id=\"caption-attachment-2574\" class=\"wp-caption-text\">Excellent-Performance-for-Low-quality-Samples<\/figcaption><\/figure>\n<h5><span class=\"ez-toc-section\" id=\"Higher_Molecular_Recovery_Rate\"><\/span>Higher Molecular Recovery Rate<span class=\"ez-toc-section-end\"><\/span><\/h5>\n<p>The IGT\u00ae Fast Library Prep Kit v2.0 can effectively reduce library distortion and sequence errors to ensure the high accuracy of the sequencing results.Compared to major competitors, the kit can obtain 30% to 50% higher sequencing depth after SSCS and DCS.<\/p>\n<figure id=\"attachment_2575\" aria-describedby=\"caption-attachment-2575\" style=\"width: 729px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2575\" src=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Higher-Molecular-Recovery-Rate.webp\" alt=\"Higher-Molecular-Recovery-Rate\" width=\"729\" height=\"336\" srcset=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Higher-Molecular-Recovery-Rate.webp 729w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/Higher-Molecular-Recovery-Rate-300x138.webp 300w\" sizes=\"auto, (max-width: 729px) 100vw, 729px\" \/><figcaption id=\"caption-attachment-2575\" class=\"wp-caption-text\">Higher-Molecular-Recovery-Rate<\/figcaption><\/figure>\n<h5><span class=\"ez-toc-section\" id=\"High_Repeatability_for_Stable_Performance\"><\/span>High Repeatability for Stable Performance<span class=\"ez-toc-section-end\"><\/span><\/h5>\n<p>The repeatability test results using the IGT\u00ae Fast Library Prep Kit v2.0 showed that the mutation rates detected (VAF \u2265 0.5%) has a superior consistency, indicating the kit is able to achieve accurate and reliable results.<\/p>\n<figure id=\"attachment_2576\" aria-describedby=\"caption-attachment-2576\" style=\"width: 832px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2576\" src=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/high-repeatability-for-stable-performance.webp\" alt=\"high-repeatability-for-stable-performance\" width=\"832\" height=\"382\" srcset=\"https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/high-repeatability-for-stable-performance.webp 832w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/high-repeatability-for-stable-performance-300x138.webp 300w, https:\/\/bio-pal.com\/eng\/wp-content\/uploads\/2025\/10\/high-repeatability-for-stable-performance-768x353.webp 768w\" sizes=\"auto, (max-width: 832px) 100vw, 832px\" \/><figcaption id=\"caption-attachment-2576\" class=\"wp-caption-text\">high-repeatability-for-stable-performance<\/figcaption><\/figure>\n<h5><span class=\"ez-toc-section\" id=\"High_Accuracy_for_Low-Frequency_Mutation_Detection\"><\/span>High Accuracy for Low-Frequency Mutation Detection<span class=\"ez-toc-section-end\"><\/span><\/h5>\n<p>With UMI-UDI adapters, the IGT\u00ae Fast Library Prep Kit v2.0 can effectively be used for low-frequency mutation tests.Tests using reference standard with known 8 mutations with VAF of 0.5% showed that all 8 mutations were successfully detected with highly accurate VAFs.<\/p>\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr class=\"firstRow\">\n<td style=\"width: 20.0138%; background-color: #41ab3c;\"><strong>Gene<\/strong><\/td>\n<td style=\"width: 20.0138%;\">Variant(AA)<\/td>\n<td style=\"width: 20.0138%;\">Expected allelic frequency(%)<\/td>\n<td style=\"width: 20.0138%;\">Repeat 1 Vaf<\/td>\n<td style=\"width: 19.945%;\">Repeat 2 Vaf<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>EGFR<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>L858R<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.50%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.40%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.47%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>EGFR<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>\u0394E746-A750<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.50%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.55%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.48%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>EGFR<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>T790M<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.50%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.81%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.40%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>EGFR<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>V769-D77insASV<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.50%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.44%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.25%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>KRAS<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>G12D<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.65%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.72%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.70%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>KRAS<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>Q61K<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.65%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.61%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.77%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>KRAS<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>A59T<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.65%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.76%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.68%<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 20.0138%; background-color: #41ab3c;\">\n<div><strong>PIK3CA<\/strong><\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>E545K<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.65%<\/div>\n<\/td>\n<td style=\"width: 20.0138%;\">\n<div>0.45%<\/div>\n<\/td>\n<td style=\"width: 19.945%;\">\n<div>0.70%<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h5><span class=\"ez-toc-section\" id=\"Ordering_Info\"><\/span>Ordering Info<span class=\"ez-toc-section-end\"><\/span><\/h5>\n<table style=\"border-collapse: collapse; width: 100%; height: 156px;\">\n<tbody>\n<tr style=\"height: 52px;\">\n<td style=\"width: 33.3333%; height: 52px;\">Product Name<\/td>\n<td style=\"width: 33.3333%; height: 52px;\">Cat. No<\/td>\n<td style=\"width: 33.3333%; height: 52px;\">Packaging<\/td>\n<\/tr>\n<tr style=\"height: 52px;\">\n<td style=\"width: 33.3333%; height: 52px;\">IGT\u00ae Fast Library Prep Kit v2.0<\/td>\n<td style=\"width: 33.3333%; height: 52px;\">C10021<\/td>\n<td style=\"width: 33.3333%; height: 52px;\">16 rxn<\/td>\n<\/tr>\n<tr style=\"height: 52px;\">\n<td style=\"width: 33.3333%; height: 52px;\">IGT\u00ae Fast Library Prep Kit v2.0<\/td>\n<td style=\"width: 33.3333%; height: 52px;\">C10022<\/td>\n<td style=\"width: 33.3333%; height: 52px;\">96 rxn<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Fast Library Prep Kit Overview The IGT\u00ae Fast Library Prep Kit v2.0 is a rapid library construction kit for fragmented [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":2577,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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Library Prep Kit Overview The IGT\u00ae Fast Library Prep Kit v2.0 is a rapid library construction kit for fragmented 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