IGT® Enzyme Plus Library Prep Kit V3
High-Efficiency NGS Library Construction
The IGT® Enzyme Plus Library Prep Kit V3 is a next-generation sequencing (NGS) library preparation solution designed for speed, flexibility, and performance. Using an enzymatic fragmentation strategy, this kit simplifies the workflow and delivers consistent, high-quality libraries.
Key Features
- Fast and streamlined protocol: Combines DNA fragmentation, end-repair, and A-tailing in a single step. Library prep can be completed in under 2.5 hours.
- Tunable insert sizes: Fragmentation parameters can be adjusted to meet specific experimental needs.
- Broad sample compatibility: Works with genomic DNA and FFPE DNA, supporting a wide range of input amounts.
- Low artifact rate: Optimized chemistry reduces erroneous ligation and repair, improving sequencing accuracy.
- EDTA tolerance: Compatible with samples containing residual EDTA.
- Multiplex-ready: Fully compatible with IGT® Adapters and indexed primers for high-throughput sequencing on platforms like Illumina and MGI.
Performance Highlights
The IGT® Enzyme Plus Library Prep Kit V3 is compatible with different DNA inputs for library construction. Following the instruction for recommended PCR cycles, you can easily generate library with high yield and stable size.
Tunable Insert Size
The IGT® Enzyme Plus Library Prep Kit V3 is capable to generate desired library inset size by simply varying the enzymatic fragmentation time according to different requirements. The library yield is stable and sufficient for subsequent experiments.
Tolerance to EDTA
The IGT® Enzyme Plus Library Prep Kit V3 could tolerate EDTA with final concentration of 0.2 mM, which will have limited influence on enzymatic fragmentation. For higher EDTA concentration, we provide Enhancer Buffer E v3 for neutralization and you may use it according to the protocol instruction.
Lower Artifact Rate
The IGT® Enzyme Plus Library Prep Kit V3 could have a lower artifact rate compared to other commercial available products, which significantly preserve the sequencing efficiency and accuracy.
Accurate Detection of Mutations
According to the analysis of detected mutations of NA12878 reference standard samples, the results were highly consistent among repetitions of library construction using IGT® Enzyme Plus Library Prep Kit V3, indicating its high accuracy.
Table. Comparison of variation detection results of cell line NA12878 sample among repetitions
| Total Mutations | Consistent Mutations | Accuracy(%) | Sensitivity(%) | Consistency Rate(%) | |
|
Repeat 1
|
17980
|
17743
|
99.26
|
99.44
|
98.70
|
|
Repeat 2
|
17977
|
17750
|
99.22
|
99.45
|
98.68
|
|
Repeat 3
|
17963
|
17744
|
99.29
|
99.43
|
98.74
|
Note: library preparation was performed by using NA12878 reference standard samples with T600V1 probe for target capture. Sequencing was performed on NovaSeq 6000 with PE150. The average sequencing depth for analysis is 139X. By using GATK, the mutations detection consistency were analyzed between the captured targeted regions and database.
Excellent Performance for Target Enrichment
The IGT® Enzyme Plus Library Prep Kit V3 can be used with hybridization-based capture workflow. The tests with TargetSeq® panels of different panel sizes showed excellent performance in capture rate, coverage rate and uniformity.
🛒 Ordering Information
| Product Name | Catalog No. | Packaging |
| IGT® Enzyme Plus Library Prep Kit V3 | C11111 | 16 rxn |
| C11112 | 96 rxn | |
| C11113 | 960 rxn |
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